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Epic Test Code LAB4848 Trichomonas vaginalis by Nucleic Acid Amplification

Performing Location(s)

BMC

Reporting Name

Trichomonas Probe

Required Specimen

  • Swab specimen must be clinician-collected using an APTIMA Collection Unisex Swab (blue) or PreservCyt Liquid PAP transfer to Aptima Specimen Transfer Kit

            Specimen Type: Cervix/Endocervix/Vaginal, Urethral (Physician Collected)

            Swab: Aptima Unisex Swab Collection Kit

  • ThinPrep Pap Test vials containing PreservCyt Solution transfer 1 ml to Aptima Specimen Transfer Kit

Specimen Type: Cervical (endocervical or ectocervical) within 30 days of collection

(Room Temperature storage of ThinPrep vials in Pathology Department)

Specimen Type

Varies

Specimen Stability

 

Specimen Type

Temperature

Time

Varies - Swab

Refrigerated (preferred)

60 days

 

Frozen

24 months

 

Ambient

60 days

PreservCyt Solution

Refrigerated (preferred)

30 days

(PAP)

Frozen

24 months

 

Ambient

14 days

Report Available

3-5 days

Causes for Rejection

  • Male patient; if male specimen is received use EPIC code LAB4848M (MML code TVRNA) for testing.  
  • urine specimen: lab will forward to Mayo clinic for testing
  • specimen collected into a SurePath device
  • transport tubes containing a cleaning swab or more than 1 swab
  • Mislabeled or unlabeled specimen
  • Aptima Collection Vaginal swab (Pink)
  • transport tubes containing a cleaning swab (white) or more than 1 swab (blue and white)
  • <1ml liquid in vial
  • Non Aptima transport tube
  • Non PreservCyt Liquid PAP transport solution
  • No swab submitted in Aptima Collection Unisex Swab vial

Specimen Retention Time

7 days

Limitations

Trichomonas: Assay detects T. vaginalis rRNA.  Although Trichomonas can be detected on wet mount, the sensitivity of wet mount is approximately 50% and dependent upon timing and the experience of the microscopist. The sensitivity of nucleic acid testing is ~95%.  For patients with clinical suspicion and a negative wet mount, this test may be beneficial.

  • Results may be affected by improper specimen collection, storage or processing. Please follow appropriate specimen collection techniques
  • Therapeutic failure or success cannot be determined with this test since nucleic acid may persist following appropriate antimicrobial therapy      
  •  A negative result does not preclude a possible infection  
  • Performance has not validated in pregnant women or women <14 years of age
  • T. tenax (commensal of oral cavity) and P. hominis (commensal of intestinal tract) may interfere and affect the ability to detect T. vaginalis

 

Reference Values

Negative

Days of Analysis

M,W,F

Methodology

Transcription mediated Amplification

Nucleic Acid Amplification test

CPT Code

87661

Last Updated

7-Oct-20