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HelpEpic Test Code LAB52 Bilirubin: Direct
Performing Location(s)
BMC,FOX,FTT,CRH,LFH,OCH,FCC,HRK
Specimen Type
Serum or Plasma
Preferred Container
Gold or Red top tube
Alternate Container
Green top
Blood Tube Draw Volume
Min 50% draw volume
Minimum Volume to Submit for Testing
0.5 mL Serum or Plasma
- Multiple test can be performed from this volume. For question please contact the lab at 547-3975.
Storage Requirements
Bilirubin is extremely photosensitive. A 50% decrease in bilirubin within 1 hour has been reported for samples exposed to direct sunlight. For optimal stability, serum samples should be stored in the darkness (or wrapped in foil) at low temperatures refrigerated
Sample tubes should be centrifuged within 2 hours of collection
Protect from light, stable 8 hours unprotected
Gold tops are stable in original tubes for 7 days DO NOT ALIQUOT
Aliquot Red tops
See Causes of Rejection for temperature requirements.
Transportation Needs
Deliver specimen to laboratory within 1 hour of collection if unspun. If not possible see "storage Requirements".
Causes for Rejection
Mislabeled or unlabeled specimen
Less than 50% draw for Vacutainer tubes|
>3 days refrigerated
> 24 hrs Room Temperature
>3 months Frozen
Hemolysis > 25 mg/dL HgB
Lipemia > 500 mg/dL Trig
Protect from light, stable 8 hours unprotected
Hemolysis criteria does not apply if ordered as PBIL
Limitations
Bilirubin is extremely photosensitive. A 50% decrease in bilirubin within 1 hour has been reported for samples exposed to direct sunlight.
Hemolyzed specimens are unacceptable for direct bilirubin testing.
See chart for limitations
| Hemoglobin (mg/dL) | Direct Bilirubin (mg/dL) | ||
|
0 |
0.3 |
3.2 |
4.3 |
|
50 |
0.3 |
3.1 |
4.1 |
|
100 |
0.2 |
2.9 |
3.9 |
|
250 |
0.2 |
2.8 |
3.4 |
|
500 |
0.1 |
2.3 |
2.8 |
Reference Values
| Population | Units | Reference Range |
| All Populations | mg/dL | 0.0-0.2 |
Available STAT
Yes
Includes
Measurement of mono- and diglucuronides and albumin complexed bilirubin
Methodology
Beckman AU/Dx Series
CPT Code
82248
Days of Analysis
All
Last Updated
26-OCT-24 MS