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Tissue Wound Culture

Collection and Handling

Microbiology Specimen Collection Procdure

Wound and Tissue Culture Specimen

Tissues

Last Updated: 03/15/2012

 

Wound/ Tissue Type

 Transport device Frequency of Culture/Replica Limits Comments
Tissue

sterile specimen cup

(none)

 Never submit a swab that has simply been rubbed over the surface.
Collection: 
1. Collect aseptically
2. Always submit as much tissue as possible.

 

 

 

General Considerations

 

  1. An order for wound culture normally includes:
    1. An Aerobic Culture
    2. A Gram Stain if adequate specimen is submitted (2 swabs, eswabs or specimen in sterile container)
    3. An Anaerobic Culture if:
      1. a portion of specimen is submitted in an anaerobic transport container: A.C.T. II Dual, eswab or red top tube
      2. the source is appropriate