Endocervical Brush/Spatula Protocol

• Obtain an adequate sampling from the ectocervix using a plastic spatula.
• Rinse the spatula as quickly as possible into the PreservCyt Solution vial by swirling the spatula vigorously in the vial 10 times.  Discard the spatula.
• Obtain an adequate sampling from the endocervix using an endocervical brush device.  Insert the brush into the cervix until only the bottom-most fibers are exposed.  Slowly rotate 1/4 or 1/2 turn in one direction.  Do Not Over-Rotate.
• Rinse the brush as quickly as possible in the PreservCyt Solution by rotating the device in the solution 10 times while pushing against the PreservCyt vial wall.  Swirl the brush vigorously to further release material.  Discard the brush.
• Tighten the cap so that the torque line on the cap passes the torque line on the vial.
  • Note: The specimen container lids must be secure to prevent leakage and contamination of the label while in transit, which would make them illegible and unable to be processed
• Record ...
  • the patient's name and ID number on the vial
  • the patient information and medical history on the Cytology requisition.
• Place the vial and requisition in a specimen bag for transport to the laboratory.