CSF Specimen

For hospitalized patients with a reported current diagnosis of viral encephalitis, CSF will be tested by molecular detection methods for a panel of viruses that include adenovirus, enterovirus, HSV 1 and 2, VZV, CMV, EBV, and HHV6.

From June through November, molecular testing will also include the following arboviruses: WNV, SLE, EEE, Cache Valley, and California serogroup viruses. Enzyme-linked immunoassay (ELISA) for WNV is performed year round on all specimens submitted with sufficient quantity.

CSF submitted on patients no longer hospitalized or with a reported current diagnosis of viral meningitis will be tested by ELISA for WNV only. Collect at least 1.0ml of CSF within seven days of onset, in a sterile, labeled container and freeze immediately at -70ºC. Ship frozen specimen overnight on a minimum of 5 lbs (2+ Kg) solid dry ice or more for multiple samples. CSF must be received frozen! For additional information regarding the viral encephalitis testing program, please visit our website at

http://www.wadsworth.org/divisions/infdis/enceph/index.htm

If less than 1.0ml of CSF is submitted, the quantity will not be sufficient to perform both ELISA and PCR testing.
1. CSF on patients with viral meningitis will be tested for WNV by ELISA (PCR will not be done since it is less
sensitive for WNV.)
2. CSF on patients with viral encephalitis will be tested according to the physician’s preference below.

Please consider the following in determining which test is most appropriate for your patient:

• ELISA is more sensitive than PCR for WNV testing and should be considered when there is a stronger suspicion of WNV than other viruses.
• PCR is less sensitive for WNV, but tests for a wide range of viruses. PCR should be considered if viruses other than WNV are suspected.

IF THERE IS LESS THAN 1.0ML OF CSF, PLEASE INDICATE TEST PREFERENCE ON REQUISTION.