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HelpEpic Test Code LAB1177 Platelet Associated Antibodies, Direct Assay
Additional Codes
ARUP:0095614
Performing Location(s)
ARUP
Ordering Recommendation
Support the diagnosis of autoimmune thrombocytopenia (AITP).
Specimen Type
Lavender (EDTA) whole blood
Preferred Container
Lavender (EDTA)
Minimum Volume
4 mL whole blood
Specimen Stability
Ambient: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Storage Requirements
CRITICAL ROOM TEMPERATURE
Transportation Needs
CRITICAL ROOM TEMPERATURE
Causes for Rejection
Mislabeled or unlabeled specimen.
Less than 50% draw for Vacutainer tubes
Specimens older than 48 hours
Clotted
hemolyzed
frozen
refrigerated specimens.
Refrance Ranges
IgG: Negative
IgM: Negative
Interpretive Data
Negative (IgG & IgM): No excess antibodies were associated with the patient's platelets. An immune cause of thrombocytopenia is unlikely.
Positive (IgG and/or IgM): An increase in platelet associated immunoglobulin is noted. An immune cause of thrombocytopenia should be considered. However, many conditions can result in an increase in platelet associated antibodies; for example, IgM rheumatoid factor antibodies.
Strong Positive (IgG and/or IgM): A definite increase in platelet associated immunoglobulin is noted and an immune cause of thrombocytopenia should be considered. However, many conditions can result in an increase in platelet associated antibodies; for example, IgM rheumatoid factor antibodies.
Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.
Limitations
Detection of platelet-associated IgG and/or IgM may be used to separate thrombocytopenia of immune origin from nonimmune origin. Most patients with ITP have abnormally high levels of IgG associated with their platelets. Occasionally patients will have normal IgG levels but abnormally high levels of IgM. Dual staining and flow cytometric analysis ensures that only platelets are analyzed and relatively small volumes of blood are required. This assay does not distinguish between autoantibodies and alloantibodies, nor does it identify specific types of antiplatelet antibodies, such as those against HPA-1a. Refer to Platelet Antibodies, Indirect (ARUP test code 0051050).
Turnaround Time
2-3 days After being received at testing Lab.
Days of Analysis
Sun-Sat
Available STAT
No
Methodolgy
Qualitative Flow Cytometry
CPT Code
86023 x2
Last Updated
2-Oct-20