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Epic Test Code LAB134 Triglycerides, Serum

Performing Location(s)

BMC

Specimen Type

Serum or Plasma

Preferred Container

Gold or Red top tube

Alternate Container

Green top tube

Minimum Volume to Submit for Testing

0.5 mL Serum or Plasma

  • Multiple test can be performed from this volume. For question please contact the lab at 547-3975.  

Storage Requirements

Sample tubes should be centrifuged within 2 hours of collection 

Gold tops are stable in original tubes for 7 days DO NOT ALIQUOT

Aliquot Red tops

See Causes of Rejection for temperature requirements.

Transportation Needs

 Deliver specimen to laboratory within 1 hour of collection if unspun. If not possible see "storage Requirements". 

Causes for Rejection

Mislabeled or unlabeled specimen
Less than 50% draw for Vacutainer tubes
> 7 days Refrigerated
> 24 hours Room Temp
>3 months Frozen
Hemolysis > 500 mg/dL HgB
Bilirubin > 40 mg/dL Bili

Limitations

Small amounts of free glycerol may be found in blood samples from healthy individuals due to natural lipolysis. The concentration of free glycerol may be increased by stress, disease states or administration of intravenous infusates. Free glycerol or other polyols may cause a positive interference.

 

N-Acetylcystein (N-AC) interference with this method leading to falsely depresses values. Collecting blood work prior to drug administration and waiting ≥12 hrs for repeat testing is recommended.

Reference Values

Population Units Reference Range
All populations mg/dL <150

 

Days of Analysis

All

Available STAT

No

Methodology

Beckman AU/Dx Series

Additional Information

It is preferred but not required that the patient be fasting for 12-14 hours prior to specimen collection

Patient Instructions for Fasting Laboratory Testing

Additional Information:

Triglycerides are water-insoluble lipids consisting of three fatty acids linked to one glycerol molecule. Triglycerides are transported in the blood as core constituents of all lipoproteins, but the greatest concentration of these molecules is carried in the triglycerides-rich chylomicrons and very low density lipoproteins (VLDL). Through the action of lipases and bile acids, triglycerides are hydrolyzed into glycerol and fatty acids which are absorbed by adipose tissue for storage or by other tissues requiring a source of energy. A peak concentration of chylomicron-associated triglycerides occurs within 3-6 hours after ingestion of a fat-rich meal; however, the rate of absorption of fats is highly variable, depending on the individual and dietary composition of the fat. After absorption, triglycerides are resynthesized in the epithelial cells and combined with cholesterol and a number of apolipoproteins to form chylomicrons

 

This test is not available on a STAT basis. If an emergency request is necessary, call 3731 for availability. Pathologist approval may be required.

 

See also entry to “Lipid Profile

CPT Code

84478

Last Updated

26-OCT-24 MS