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Epic Test Code LAB21130 Hepatitis C Total Antibody

Performing Location(s)

BMC

Synonyms

Antibody to Hepatitis C, or Non-A, Non-B Hepatitis

Specimen Type

Serum

Preferred Container

Gold or Red top

Blood Tube Draw Volume

Min 50% draw volume  

Minimum Volume to Submit for Testing

2 mL Serum

  • Multiple test can be performed from this volume. For question please contact the lab at 607-547-3975.  

Storage Requirements

Primary specimen tubes should be centrifuged within 2 hours of collection followed by transfer of the serum to a labeled plastic, aliquot tube.

See Causes of Rejection for temperature requirements.

Transportation Needs

Deliver specimen to laboratory within 1 hour of collection if unspun. If not possible see "storage Requirements". 

Causes for Rejection

Mislabeled or unlabeled specimen
Less than 50% draw for Vacutainer tubes
Plasma

Spun or Unspun sample >7 days at 2-8 °C

Spun or Unspun sample >3 days at Room Temperature

Hemolysis > 500 mg/dL HgB
Lipemia > 3000 mg/dL Trig
Bilirubin > 20 mg/dL Bili
Total Protein >12 g/dL

EDTA, Heparin, NaF, Citrate, Oxalate

 

Limitations

Assay is limited to detection of IgG antibodies to Hepatitis C

A negative result does not exclude the possibility of exposure to Hepatitis C virus

A positive result does not exclude co-infection by another hepatitis virus

Assay results above the cut-off are not indicative of antibody level

Performance of this assay has not been established with cord blood, neonatal specimens, cadaver specimens or other body fluids

Reference Values

 

In response to the MMWR's Guidelines for Laboratory Reporting of Antibody to Hepatitis C Virus (Volume 152 2/7/2003), the testing and reporting protocol for Hepatitis C  is as follows:
 

Value S/CO

Reported as:

Reflexive Action

<0.80

Negative

None

0.80 – 0.99

Equivocal

A confirmatory Hepatitis C Virus RNA by RT- PCR(Reference Lab test LAB887 HCQTR) is required. and has been reflexed. If needed, the laboratory will contact patient for redraw.

≥1.00

 Presumptive Positive

Presumptive Hepatitis C infection. Consistent with current HCV or past infection that was resolved or biologic false positive .A confirmatory Hepatitis C Virus RNA by RT- PCR(Reference Lab test LAB887 HCQTR) is required. and has been reflexed. If needed, the laboratory will contact patient for redraw.Order Lab887 to identify current infection.

 

 

Reflex Testing

Equivocal and Preumptive positive results will refelx to LAB887 Hepatitis C Virus (HCV) RNA Detection and Quantification by Real-Time Reverse Transcription-PCR (RT-PCR), Serum. CPT Code 87522

 

 

Includes

Qualitative analysis for Hepatitis C Antibody; See also Reflexive Testing below

Available STAT

No

Days of Analysis

All

Additional Information

Hepatitis C is a NYSDOH reportable disease. The NYSDOH will be notified of all positive cases

This test is not available on a STAT basis or off-hours, weekends or holidays. If an emergency request is necessary, call 3731 for availability. Pathologist approval may be required.

 

Hepatitis Testing Guideline/Information

Methodology

Chemilumimetric Immunoassay

CPT Code

86803

Last Updated

7-Dec-22 BHD