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Epic Test Code LAB2295 Mononucleosis with Reflex to EBVC

Performing Location(s)

BMC,CRH,LFH,OCH,FTT,FOX 

Synonyms

Monospot, Mono test

Specimen Type

Serum

Preferred Container

Gold or Red top tube

Blood Tube Draw Volume

Min 50% draw volume  

Minumum Volume to Submit for Testing

1 mL Serum

  • Multiple test can be performed from this volume. For question please contact the lab at 547-3975.  

Storage Requirements

  • Specimens being transported to the lab via packing list must be frozen for transport.
  • Gold top or red top tubes should be centrifuged within 2 hours of collection.
  • Serum samples must be aliquated tested within 1 hour of centrifugation. If testing cannot be performed within 1 hour of centrifugation, aliquated specimen must be refrigerated until testing is performed.
  • Specimens may be refrigerated (2-8⁰C) for up to 48 hours or frozen (-10⁰ C) for up to 3 months.
  • Specimens must be at room temperature (15-30⁰ C) when tested.

 

Transportation Needs

  •  Deliver specimen to laboratory within 1 hour of collection if unspun. If not possible see "storage Requirements". 
  • Aliquoted specimens must be frozen within 1 hour for transport.

Causes for Rejection

Mislabeled or unlabeled specimen

Less than 50% draw for Vacutainer tubes

Serum samples > 1 hour at room temperature

Serum samples > 48 hours old, refrigerated

Serum > 3 month, Frozen

Clotted anticoagulated specimens

Unspun whole blood samples > 24 hours old at Room Temperature

Limitations

Interpret results along with clinical symptoms. This kit tests for IgM heterophile antibodies False positive results are known to occur due to cross-reactivity from the presence of rheumatoid factor. False negative results may occur from the timing of specimen collection relative to antibody titer peaks and troughs. A negative result should be supplemented with EBV specific serology if EBV infection is suspected.

 

As in the case of other diagnostic procedures, the results obtained by the use of this kit yield data that must be evaluated by the physician in light of other clinical information.

 

Although most patients will have a detectable heterophile level within three weeks of infection, occasionally a patient with strong clinical signs of infectious mononucleosis may take as long as three months to develop a detectable level. If further testing is desired, collect additional specimens and retest every few weeks

 

IM heterophile has been associated with disease states other than infectious mononucleosis, such as leukemia, Epstein-Barr virus, cytomeglovirus, Burkitt’s lymphoma, rheumatoid arthritis, and viral hepatitis.

Reference Values

Population Units Reference Range
All Populations none Negative

 

Reflex Testing

When results are Negative testing will reflex to LAB2457 Epstein-Barr Virus (EBV) Antibody Profile, Serum.

Days of Analysis

All

Available STAT

No

Additional Information

Additional Information: A CBC should exhibit atpical lymphocytes if patient has mononucleosis

 

Methodology

Rapid Immunochromatographic

CPT Code

86308

Last Updated

18-Mar-22 KT