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Epic Test Code LAB2910 Phenosense Combination HIV Drug Resistance Assay

Additional Codes

MML:FPGT

Reporting Name

Phenosense GT

Performing Laboratory

Monogram Biosciences, Inc

Specimen Type

Plasma EDTA


Ordering Guidance


This procedure should be used for patients with documented HIV-1 infection and viral loads =500 copies/mL.



Specimen Required


Specimen Type: Plasma

Container/Tube: Lavender-top (EDTA) or plasma preparation tube (PPT)

Specimen Volume: 3 mL

Collection Instructions: Draw blood in either PPT (pearl top) or lavender-top (EDTA) tubes. Centrifuge specimen within six hours of collection. Remove plasma from cells immediately, and transfer specimen to a screw-capped, plastic vial. Freeze 3 mL of PPT plasma or EDTA plasma immediately, send specimen frozen.

 

To avoid delays in turnaround time when requesting multiple tests, please submit separate frozen specimens for each test requested.

 

RECOMMENDED:

1.     Patient's most recent viral load.

2.     Viral load collection date.

 

NOTE:  1. Intended for use only for patients with viral loads greater than or equal to 500 copies/mL. For best results, viral loads should be confirmed within 2 weeks prior to submission for testing at Monogram.

            2. Patient samples submitted <30 days apart are considered duplicate and will be cancelled.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Frozen 14 days

Reference Values

A final report will be attached in MayoAccess.

 

Day(s) Performed

Sunday through Saturday

CPT Code Information

87900/Infectious agent drug susceptibility phenotype prediction

87901/Infectious agent genotype analysis by nucleic acid; reverse transcriptase and protease

87903/Infectious agent phenotype analysis by nucleic acid with drug resistance tissue culture analysis; first through 10 drugs tested

87904/x12 Each additional drug tested

Report Available

18 to 26 days

Reject Due To

Other PPT(TM) not centrifuged; quantity not sufficient for analysis; heparinized plasma; nonfrozen specimen; specimen exposed to repeated freeze/thaw cycles; specimen received in "pop-top" or "snap-cap" tube

NY State Approved

Yes

Method Name

Phenotype: polymerase chain reaction (PCR) amplification and viral culture; genotype: polymerase chain reaction (PCR) and DNA sequencing.

Useful For

Determines viral phenotype and genotype resistance to three classes of commonly prescribed antiretroviral drugs: nucleoside reverse transcriptase inhibitors (NRTI), nonnucleoside reverse transcriptase inhibitors (NNRTI), and protease inhibitors (PI). Viral replication capacity is also included.