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Epic Test Code LAB303 Coagulation Factor II Activity Assay, Plasma

Additional Codes

MML Code: F_2

 

NY State Approved

Yes

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Reporting Name

Coag Factor II Assay, P

Method Name

Optical Clot-Based

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen 14 days


Ordering Guidance


Coagulation testing is highly complex, often requiring the performance of multiple assays and correlation with clinical information. For that reason, we suggest ordering Coagulation Consultations.



Necessary Information


If priority specimen, mark request form, give reason, and request a call-back.



Specimen Required


Specimen Type: Platelet-poor plasma

Patient Preparation:  Patient must not be receiving coumadin (warfarin) or heparin therapy. (If not possible for medical reasons, note on request.)

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Specimen must be collected prior to factor replacement therapy

2. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing

3. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

4. Aliquot plasma into a plastic vial, leaving 0.25 mL in the bottom of centrifuged vial.

5. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or, ideally, -40° C or below.

 

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.


Bassett Healthcare Network Clinical Laboratories Note:

Blood Tube Draw Volume
Min 90% draw volume  

Specimen Type

Plasma Na Cit

Specimen Minimum Volume

0.5 mL

Reference Values

Adults: 75-145%

Normal, full-term newborn infants or healthy premature infants may have decreased levels (≥25%) which may remain below adult levels for ≥180 days postnatal.*

 

*See Pediatric Hemostasis References section in Coagulation Guidelines for Specimen Handling and Processing

Report Available

1 to 3 days

Day(s) Performed

Monday through Saturday

CPT Code Information

85210

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Useful For

Diagnosing a congenital deficiency (rare) of coagulation factor II

 

Evaluating acquired deficiencies associated with liver disease or vitamin K deficiency, oral anticoagulant therapy, and antibody-induced deficiencies (eg, in association with lupus-like anticoagulant)

 

Determining warfarin treatment stabilization in patients with nonspecific inhibitors (ie, lupus anticoagulant)

 

Determining degree of anticoagulation with warfarin to correlate with level of protein S

 

Investigation of prolonged prothrombin time or activated partial thromboplastin time

Clinical Information

Factor II (prothrombin) is a vitamin K-dependent serine protease synthesized in liver. It participates in the final common pathway of coagulation, as the substrate for the prothrombinase enzyme complex. Prothrombin is the precursor of thrombin (IIa), which converts fibrinogen to fibrin. Plasma biological half-life is about 3 days.

 

Deficiency of factor II may cause prolonged prothrombin time and activated partial thromboplastin time. Deficiency may result in a bleeding diathesis.

Interpretation

Liver disease, vitamin K deficiency, or warfarin anticoagulation can cause decreased factor II activity.

 

Normal newborn infants may have levels of 25% to 50%.

Cautions

Factor II is one of the last vitamin K-dependent coagulation factors to decrease after starting warfarin therapy and one of the last to return to normal when anticoagulation is discontinued. It may take 10 to 14 days for a return to baseline levels.

Specimen Retention Time

7 days