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Epic Test Code LAB308 Coagulation Factor IX Activity Assay, Plasma

Additional Codes

MML Code: F_9

 

NY State Approved

Yes

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Reporting Name

Coag Factor IX Assay, P

Method Name

Optical Clot-Based

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen 14 days


Ordering Guidance


Coagulation testing is highly complex, often requiring the performance of multiple assays and correlation with clinical information. For that reason, consider ordering a Coagulation Consultation.



Necessary Information


If priority specimen, mark request form, give reason, and request a call-back.



Specimen Required


Specimen Type: Platelet-poor plasma

Patient Preparation: Patient must not be receiving Coumadin (warfarin) or heparin therapy.

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Specimen must be collected prior to factor replacement therapy.

2. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.

3. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

4. Aliquot plasma into a plastic vial, leaving 0.25 mL in the bottom of centrifuged vial.

5. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or ideally, at or below -40° C.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.


Bassett Healthcare Network Clinical Laboratories Note:

Blood Tube Draw Volume
Min 90% draw volume  

Specimen Type

Plasma Na Cit

Specimen Minimum Volume

0.5 mL

Reference Values

≤6 months: Normal, full-term newborn infants or healthy premature infants may have decreased levels (≥20%), which may not reach adult levels for 180 or more days postnatal.* (Literature derived)

>6 months: 65-140%

*See Pediatric Hemostasis References section in Coagulation Guidelines for Specimen Handling and Processing.

Report Available

1 to 3 days

Day(s) Performed

Monday through Saturday

CPT Code Information

85250

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Useful For

Diagnosing deficiencies, particularly hemophilia B (Christmas disease)

 

Assessing the impact of liver disease on hemostasis

 

Investigation of a prolonged activated partial thromboplastin time

Disease States

  • Hemophilia B

Clinical Information

Factor IX is a vitamin K-dependent serine protease synthesized in the liver and participates in the intrinsic coagulation pathway. Its biological half-life is 18 to 24 hours.

 

Congenital deficiency is inherited as an X-linked recessive bleeding disorder (hemophilia B). Severe deficiency (<1%) is characterized by hemarthroses, deep tissue bleeding, excessive bleeding with trauma, and ecchymoses.

 

Acquired deficiency is associated with liver disease, vitamin K deficiency, warfarin therapy, and inhibitors (rare).

Interpretation

Acquired deficiency is more common than congenital.

 

Mild hemophilia B: 5% to 50% activity

 

Moderate hemophilia B: 1% to 5% activity

 

Severe hemophilia B: <1% activity

Cautions

Liver disease, warfarin therapy, or vitamin K deficiency may decrease factor IX levels.

Specimen Retention Time

7 days

Testing Algorithm

For information see Hemophilia Testing Algorithm