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Epic Test Code LAB310 Coagulation Factor XII Activity Assay, Plasma

Additional Codes

MML Code: F_12

 

NY State Approved

Yes

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Reporting Name

Coag Factor XII Assay, P

Method Name

Optical Clot-Based

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen 14 days


Ordering Guidance


Coagulation testing is highly complex, often requiring the performance of multiple assays and correlation with clinical information. For that reason, consider ordering a Coagulation Consultation.



Necessary Information


If priority specimen, mark request form, give reason, and request a call-back.



Specimen Required


Specimen Type: Platelet-poor plasma

Patient Preparation: Patient must not be receiving Coumadin (warfarin) or heparin therapy.

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Specimen must be collected prior to factor replacement therapy

2. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.

3. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

4. Aliquot plasma into a plastic vial, leaving 0.25 mL in the bottom of centrifuged vial.

5. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or ideally, at or below -40° C.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.


Bassett Healthcare Network Clinical Laboratories Note:

Blood Tube Draw Volume
Min 90% draw volume  

Specimen Type

Plasma Na Cit

Specimen Minimum Volume

0.5 mL

Reference Values

Adults: 55-180%

Normal, full-term newborn infants or healthy premature infants may have decreased levels (≥15% to 20%), which may not reach adult levels for 180 or more days postnatal.*

*See Pediatric Hemostasis References section in Coagulation Guidelines for Specimen Handling and Processing.

Report Available

1 to 3 days

Day(s) Performed

Monday through Saturday

CPT Code Information

85280

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Useful For

Diagnosing deficiency of coagulation factor XII

 

Determining cause of prolonged activated partial thromboplastin time

Clinical Information

Factor XII is synthesized in the liver. Its biological half-life is 40 to 50 hours. Factor XII is a component of the contact activation system and is involved in both intrinsic pathway and fibrinolytic system.

 

Factor XII deficiency is often discovered when activated partial thromboplastin time is found to be unexpectedly long. The deficiency does not cause a known bleeding disorder.

 

An association between severe factor XII deficiency and thrombosis risk has been proposed but not proven.

Interpretation

Acquired deficiency is associated with liver disease, nephritic syndrome, and chronic granulocytic leukemia.

 

Congenital homozygous deficiency: 20% activity

 

Congenital heterozygous deficiency: 20% to 50% activity

Cautions

Deficiencies of other contact activator proteins (prekallikrein, high molecular weight kininogen) can also cause prolonged activated partial thromboplastin time but do not cause clinical bleeding.

Specimen Retention Time

7 days