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HelpEpic Test Code LAB472 Hepatitis B Surface Antibody
Performing Location(s)
BMC
Synonyms
Anti-HBs
Specimen Type
Serum
Preferred Container
Gold or Red top
Blood Tube Draw Volume
Min 50% draw volume
Minimum Volume to Submit for Testing
2 mL Serum
- Multiple test can be performed from this volume. For question please contact the lab at 547-3975.
Transportation Needs
Deliver specimen to laboratory within 1 hour of collection if unspun. If not possible see "storage Requirements".
Storage Requirements
Primary specimen tubes should be centrifuged within 2 hours of collection followed by transfer of the serum (red or gold top) to a labeled plastic, aliquot tube.
See Causes of Rejection for temperature requirements.
Causes for Rejection
Mislabeled or unlabeled specimen
Less than 50% draw for Vacutainer tubes
Plasma
Spun or Unspun sample >7 days at 2-8 °C
Spun or Unspun sample >3 days at Room Temperature
Hemolysis > 500 mg/dL HgB
Lipemia > 3000 mg/dL Trig
Bilirubin > 20 mg/dL Bili
Total Protein >12 g/dL
EDTA, Heparin, NaF, Citrate, Oxalate
Limitations
This assay does not differentiate between a vaccine-induced immune response and immune response induced by infection with HBV. To determine if anti-HBs response is due to vaccine or HBV infection, a total anti-HBc assay may be performed
Performance of this assay has not been established with cord blood, cadaver specimens or other body fluids.
A positive result does not exclude co-infection by another hepatitis virus.
A reactive result does not exclude co-infection by other hepatitis virus.
Results from immunocompromised patients should be interpreted with caution.
Reference Values
| Result | Status | Interpretation |
| <8.00 mIU/mL | Negative |
No protective immunity to HBV |
| ≥12.00 mIU/mL | Positive |
Protective immunity to HBV infection |
Includes
Anti HBS Concentration and interpretive chart
Days of Analysis
M-F only.
Available STAT
No
Additional Information
This test is not available on a STAT basis or off-hours, weekends or holidays. If an emergency request is necessary, call 3731 for availability. Pathologist approval may be required.
Methodology
Chemilumimetric Immunoassay
CPT Code
86706
Last Updated
18-Mar-22 BHD