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Epic Test Code LAB648 Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA with Reflex to dsDNA Antibody, IgG by IFA

Additional Codes

ARUP: 0050215

Specimen Type

Serum

Preferred Container

Gold top tube

Minumum Volume to Submit for Testing

1 mL Serum

  • Multiple test can be performed from this volume. For question please contact the lab at 547-3975.

Storage Requirements

Ambient: 48 hours
Refrigerated: 2 weeks
Frozen: 1 year (avoid repeated freeze/thaw cycles) 

Cause of Rejection

Less than 50% draw for Vacutainer tubes
Mislabeled or unlabeled specimen
Plasma.
Contaminated,
hemolyzed, 
severely lipemic specimens

Days of Analysis

Sun-Sat

Turnaround Time

1-3 Days once specimen is recived into testing lab

Methodology

Qualitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody

Reference Value

Components

Reference Interval

Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA

None Detected.

Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae)

Less than 1:10

 

Reflex Testing

If dsDNA IgG antibody is detected, the result will be confirmed by Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae). Additional charges apply.

Interpretive Data

Positivity for anti-double stranded DNA (anti-dsDNA) IgG antibody is a diagnostic criterion of systemic lupus erythematosus (SLE). Specimens are initially screened by enzyme-linked immunosorbent test (ELISA). All ELISA results reported as "detected" (positive) are confirmed by a highly specific IFA titer (Crithidia luciliae indirect fluorescent test [CLIFT]). Some patients with early or inactive SLE may be positive for anti-dsDNA IgG by ELISA but negative by CLIFT. If the patient is negative by CLIFT but positive by ELISA and clinical suspicion remains, consider antinuclear antibody (ANA) testing by IFA. Additional information and recommendations for testing may be found at http://www.arupconsult.com/Topics/AutoimmuneDz/ConnectiveTissueDz/index.html.

Ordering Recommendation 

Secondary screening for systemic lupus erythematosus (SLE) based on ANA results.

CPT Code

86225; if reflexed, add 86256

Last Updated

8-Nov-18

Transporation Needs

Deliver specimen to the laboratory within 1 hour of collection. If not possible see “storage requirements”

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. And send refigerated