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Epic Test Code LAB718 Oxyhemoglobin, Arterial (NOVABIO)

Performing Location(s)

BMC,CRH,LFH,OCH,FTT,FOX

Synonyms

Oxyhemoglobin, Oxygen Saturation

Specimen Type

Arterial Whole Blood

Preferred Container

Heparin coated syringe .

Expel all air bubbles from syringe immediately after collection.

Alternate Container

Lithium Heparin Tube

Specimen Tubes for Blood Gases

.

Volume Required

2 mL

Transportation Needs

Deliver ASAP within 15 minutes.
Do not send Green top tubes through pneumatic tube system.
Do not centrifuge.
Stable 30 minutes at RT.
Do Not Send on Ice

Storage Requirements

Deliver ASAP within 15 minutes.
Do not send Green top tubes through pneumatic tube system.
Do not centrifuge.
Stable 30 minutes at RT.
 

Causes for Rejection

Mislabeled or unlabeled specimen
Less than 95% draw for Vacutainer tubes
Specimen not received within 30 min of collection at room temp
Specimen delivered on ICE
Specimen contaminated with Room Air (bubbles in syringe or uncapped),
Clotted or Centrifuged
Pneumatic tube transport of Green top tubes
All other tubes or anticoagulant other than Lithium heparin whole blood

No Add-ons

Limitations

Errors can occur due to metabolic changes if there is a delay in the measurement of the sample.

Severely abnormal plasma osmolarities or abnormal levels of proteins or lipids can potentially interfere with sample analysis.

As with all spectrophotometric methods, there are limitations regarding the optical determination of hemoglobin derivatives. In general, any substance, which in addition the five hemoglobin derivatives to be determined, causes significant absorption or light scattering of the sample, may have a negative impact on the result.

Reference Values

Analyte Units Reference Range
Oxyhemoglobin % 95 - 98
Oxygen Content ml/dl 15 - 23

Reference

Ann Lab Med 2020;40(6): 448-56

Includes

Spectrophotometrically measured Oxyhemoglobin (O2Hb) expressed as a fraction of the total hemoglobin.

O2content is included.

Days of Analysis

All

Methodology

See testing site dependent information.
Multiple wavelength measurement of optical density to determine the concentration of [O2Hb\ (oxyhemoglobin), [HHb] (deoxyhemoglobin), [MetHb] (methemoglobin) and [COHb] (carboxyhemoglobin).

The calculation then used to calculate the O2SAT (%O2Hb) is:

%O2Hb=([O2Hb])/([O2Hb]+ [HHb]+ [MetHb]+ [COHb] )

CPT Code

82810

Last Updated

2-Mar-23 BHD