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HelpEpic Test Code LAB722 Antibody to Extractable Nuclear Antigen Evaluation, Serum
Additional Codes
MML Code: ENAE
NY State Approved
YesPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Ab to Extractable Nuclear Ag Eval,SMethod Name
Multiplex Flow Immunoassay
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 21 days | |
| Frozen | 21 days |
Specimen Required
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Blood Tube Draw Volume
Min 50% draw volume
Specimen Type
SerumSpecimen Minimum Volume
0.35 mL
Reference Values
SS-A/Ro ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
SS-B/La ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
Sm ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
RNP ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
Scl 70 ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
Jo 1 ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
Report Available
Same day/1 to 2 daysDay(s) Performed
Monday through Saturday
CPT Code Information
86235 x 6
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
| Heat-Treated | Reject |
Useful For
Evaluating patients with clinical features suggestive of antinuclear antibody (ANA) associated connective tissue disease. May also be indicated in patients who test negative for ANA and have features of Sjogren syndrome and idiopathic inflammatory myopathies
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| SSA | SS-A/Ro Ab, IgG, S | Yes | Yes |
| SSB | SS-B/La Ab, IgG, S | Yes | Yes |
| SM | Sm Ab, IgG, S | Yes | Yes |
| RNP | RNP Ab, IgG, S | Yes | Yes |
| SCL70 | Scl 70 Ab, IgG, S | Yes | Yes |
| JO1 | Jo 1 Ab, IgG, S | Yes | Yes |
Testing Algorithm
For more information see Connective Tissue Disease Cascade.
Special Instructions
Clinical Information
Antibodies to SS-A/Ro, SS-B/La, Smith (Sm), U1RNP (RNP68/A), Scl 70 and JO1 are associated with the presence of antinuclear antibodies (ANA) and useful in the evaluation of specific ANA-associated connective tissue diseases (CTD), ANA-CTD.(1) Due to their frequencies in ANA-CTD and the overlapping clinical presentations of these diseases, inclusion of these tests in a panel may be useful at initial evaluation of patients at-risk for certain CTD. The combined presence of antibodies to SS-A/Ro (Ro52 and Ro60) and anti-SS-B/La is highly suggestive of Sjogren syndrome.(2,3) Separate determination of anti-Ro52 and anti-Ro60 antibodies is preferred to combined SS-A/Ro in the evaluation of ANA-CTD as their differential presence maybe useful in risk stratification and prognosis of ANA-CTD patients.(4) The presence of anti-Sm antibodies are specific for systemic lupus erythematosus (SLE) and is included the classification criteria for disease.(5) Monospecific antibody reactivity to U1RNP may indicate a diagnosis of mixed connective tissue disease (MCTD).(6) However, anti-U1RNP antibodies may also be seen in patients with SLE, systemic sclerosis (SSc) and idiopathic inflammatory myopathies (IIM).(6,7) In addition, there exists diverse analytes for the detection U1RNP with differential correlations with MCTD, SLE, SSc, and IIM.(8) Anti-Scl 70 (topoisomerase 1) antibody is one of three autoantibodies included in the 2013 American College of Rheumatology/European League Against Rheumatism (ACR/EULAR) classification criteria for SSc.(9). It is generally associated with diffuse cutaneous SSc clinical manifestations including interstitial lung disease with poor prognostic outcomes.(10) Lastly, antibodies to JO1, is a member of the amino acyl-tRNA synthetase family of enzymes is suggestive of diagnosis of IIM, specifically anti-synthetase syndrome and is included in the 2017 EULAR/ACR classification for IIM.(11)
For more information, see the individual test codes.
Interpretation
A positive antibody result in the appropriate clinical context maybe suggestive of connective tissue disease.
For more information, see the individual test codes.
Cautions
Negative results to do not rule out the presence of connective tissue disease.