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Epic Test Code LAB727 Glomerular Basement Membrane Antibodies, IgG, Serum

Additional Codes

MML Code: GBM

LIS Code: AGBM

NY State Approved

Yes

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Reporting Name

Glomerular Basement Membrane IgG Ab

Method Name

Multiplex Flow Immunoassay

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 21 days
  Frozen  21 days


Advisory Information


If patient is being evaluated for autoimmune skin disease, order CIFS / Cutaneous Immunofluorescence Antibodies (IgG), Serum for evaluation of anti-intercellular substance (ICS) and antibasement membrane zone (BMZ) antibodies.



Specimen Required


Container/Tube: 

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


Specimen Type

Serum

Specimen Minimum Volume

0.35 mL

Reference Values

<1.0 U (negative)                     

≥1.0 U (positive)

Reference values apply to all ages.

Analytic Time

Same day/1 day

Day(s) and Time(s) Performed

Monday through Friday, 4 p.m.

CPT Code Information

83516

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

Mild OK; Gross OK

Other

NA

Cautions

A positive test for glomerular basement membrane (GBM) antibodies cannot be relied upon exclusively to establish the diagnosis of a disease mediated by GBM antibodies. Weakly-positive test results may occur in other immune-mediated diseases, and renal or lung biopsy is often required to establish the diagnosis.

 

If patient is being evaluated for autoimmune skin disease, order CIFS / Cutaneous Immunofluorescence Antibodies (IgG), Serum for evaluation of anti-intercellular substance (ICS) and antibasement membrane zone (BMZ) antibodies.

Specimen Retention Time

14 days

Clinical Information

Antibodies to glomerular basement membrane (GBM) antigens cause glomerulonephritis, Goodpasture syndrome (glomerulonephritis, often with rapid onset renal failure, and pulmonary hemorrhage), and, less commonly, pulmonary hemosiderosis.(1) Nephrogenic GBM antigens are associated with the noncollagenous carboxyl extension of type IV procollagen. The immunologic stimuli that elicit production of GBM antibodies are not known. There is some evidence of a genetic association with HLA-DR2.

 

GBM antibody-mediated glomerulonephritis and Goodpasture syndrome occur with a bimodal age distribution primarily in males ages 20 to 40 and in patients older than age 50. Glomerulonephritis without pulmonary involvement is more common in the older age group, and shows a female predominance.

Useful For

Evaluating patients with rapid onset renal failure or pulmonary hemorrhage, as an aid in the diagnosis of Goodpasture syndrome

Interpretation

Positive results are consistent with Goodpasture syndrome. Glomerular basement membrane antibodies detected by immunoassay have been reported to be highly specific for Goodpasture syndrome. The sensitivity of this test approaches 87% in untreated patients with systemic disease.(1)