Epic Test Code LAB89 Haptoglobin, Serum
Additional Codes
MML Code: HAPT
LIS Code: HPTG
NY State Approved
YesPerforming Laboratory
Mayo Clinic Laboratories in RochesterReporting Name
Haptoglobin, SMethod Name
Nephelometry
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 28 days | |
Frozen | 28 days | ||
Ambient | 14 days |
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume:1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Type
SerumSpecimen Minimum Volume
0.5 mL
Reference Values
30-200 mg/dL
Report Available
1 to 3 daysDay(s) Performed
Monday through Friday
CPT Code Information
83010
Reject Due To
Gross hemolysis | OK |
Gross lipemia | Reject |
Gross icterus | OK |
Useful For
Confirmation of intravascular hemolysis
Clinical Information
Haptoglobin is an immunoglobulin-like plasma protein that binds hemoglobin. The haptoglobin-hemoglobin complex is removed from plasma by macrophages and the hemoglobin is catabolized. When the hemoglobin-binding capacity of haptoglobin is exceeded, hemoglobin passes through the renal glomeruli, resulting in hemoglobinuria.
Chronic intravascular hemolysis causes persistently low haptoglobin concentration. Regular strenuous exercise may cause sustained low haptoglobin, presumably from low-grade hemolysis. Low serum haptoglobin may also be due to severe liver disease.
Neonatal plasma or serum specimens usually do not contain measurable haptoglobin; adult levels are achieved by 6 months.
Increase in plasma haptoglobin concentration occurs as an acute-phase reaction. Levels may appear to be increased in conditions such as burns and nephrotic syndrome. An acute-phase response may be confirmed and monitored by assay of other acute-phase reactants such as alpha-1-antitrypsin and C-reactive protein.
Interpretation
Absence of plasma haptoglobin may indicate intravascular hemolysis. However, congenital anhaptoglobinemia is common, particularly in African Americans. For this reason, it may be difficult or impossible to interpret a single measurement of plasma haptoglobin. If the assay value is low, the test should be repeated after 1 to 2 weeks following an acute episode of hemolysis. If all the plasma haptoglobin is removed following an episode of intravascular hemolysis and if hemolysis ceases, the haptoglobin concentration should return to normal in a week.
Low levels of plasma haptoglobin may indicate intravascular hemolysis.
Cautions
Low haptoglobin is normal for the first 3 to 6 months of life.
Haptoglobin is an acute-phase reactant and increases with inflammation or tissue necrosis.