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HelpEpic Test Code LAB21115 Bilirubin Fractionation
Performing Location(s)
BMC,FOX,FTT,CRH,LFH,OCH,FXCC,HRK
Specimen Type
Serum or Plasma
Preferred Container
Gold or Red top tube
Alternate Container
Green top
Blood Tube Draw Volume
Min 50% draw volume
Minimum Volume to Submit for Testing
0.5 mL Serum or Plasma
- Multiple test can be performed from this volume. For question please contact the lab at 547-3975.
Storage Requirements
Bilirubin is extremely photosensitive. A 50% decrease in bilirubin within 1 hour has been reported for samples exposed to direct sunlight. For optimal stability, serum samples should be stored in the darkness (or wrapped in foil) at low temperatures refrigerated
Microtainer tubes should be centrifuged within 2 hour of collection. Serum from microtainer collection tubes does NOT require transfer to another tube
See Causes of Rejection for temperature requirements.
Transportation Needs
Deliver specimen to laboratory within 1 hour of collection if unspun. If not possible see "storage Requirements".
Causes for Rejection
Mislabeled or unlabeled specimen
Less than 50% draw for Vacutainer tubes
>3 days refrigerated
>24 hours Room Tempature
>3 months Frozen
Hemolysis > 25 mg/dL HgB
Lipemia > 500 mg/dL Trig
Protect from light, stable 8 hours unprotected
Hemolysis criteria does not apply if ordered as Bilirubin Fractionation: Pediatric
Limitations
Bilirubin is extremely photosensitive. A 50% decrease in bilirubin within 1 hour has been reported for samples exposed to direct sunlight
Hemolyzed specimens are unacceptable for direct bilirubin testing.
See chart for limitations
| Hemoglobin (mg/dL) | Direct Bilirubin (mg/dL) | ||
|
0 |
0.3 |
3.2 |
4.3 |
|
50 |
0.3 |
3.1 |
4.1 |
|
100 |
0.2 |
2.9 |
3.9 |
|
250 |
0.2 |
2.8 |
3.4 |
|
500 |
0.1 |
2.3 |
2.8 |
Reference Values
| Analyte | Units | Population | Reference Range |
| Total Bilirubin | mg/dL | >38 days old | 0.3-1.0 |
| Direct Bilirubin | mg/dL | All Populations | 0.0-0.2 |
Includes
Total Bilirubin, Direct Bilirubin and calculated indirect bilirubin.
NOTE: this panel no longer includes conjugated bilirubin or the bilirubin-albumin complex
Available STAT
Yes
Days of Analysis
All
Methodology
Beckman AU/Dx Series (see individual tests for specific information (i.e. Total Bilirubin and Direct Bilirubin)
CPT Code
82248, 82247
Last Updated
7-Nov-23 BHD