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HelpEpic Test Code LAB51 Bilirubin Fractionation: Neonatal
Performing Location(s)
MIB,FOX,FTT,CRH,LFH,OCH,FXCC,HLS
Specimen Type
Serum or Plasma
Preferred Container
Gold Microtainer
Alternate Container
Gold, Red, or Green top tube, Green Microtainer
Minumum Volume to Submit for Testing
0.5 mL Serum or Plasma
- Multiple test can be performed from this volume. For question please contact the lab at 547-3975.
Transportation Needs
Deliver specimen to laboratory within 1 hour of collection if unspun. If not possible see "storage Requirements".
Storage Requirements
Bilirubin is extremely photosensitive. A 50% decrease in bilirubin within 1 hour has been reported for samples exposed to direct sunlight. For optimal stability, serum samples should be stored in the darkness (or wrapped in foil) at low temperatures refrigerated
Microtainer tubes should be centrifuged within 2 hours of collection. Serum from microtainer collection tubes does NOT require transfer to another tube
Sample tubes should be centrifuged within 2 hours of collection followed by transfer of the serum (red or gold top) or plasma (green top) to a labeled plastic, aliquot tube
Causes for Rejection
Mislabeled or unlabeled specimen
Less than 50% draw for Vacutainer tubes
>3 days refrigerated
> 24 hours room temperature
> 3 montsh Frozen
Protect from light, stable 8 hours unprotected
Hemolysis > 25 mg/dL HgB
Lipemia > 500 mg/dL Trig
Hemolysis criteria does not apply of ordered as a PBIL
NOTE: The clinical laboratory no longer rejects pediatric bilirubins based on hemolysis. When detected, hemolyzed specimens with resulting hemoglobin > 100 mg/dL will be resulted and “flagged” with the comment: “Specimen hemolyzed. DBIL may be underestimated” (see Limitations of Procedure)
Limitations
Bilirubin is extremely photosensitive. A 50% decrease in bilirubin within 1 hour has been reported for samples exposed to direct sunlight
Hemolyzed specimens are generally unacceptable for direct bilirubin testing
See chart for limitations
| Hemoglobin (mg/dL) | Direct Bilirubin (mg/dL) | ||
|
0 |
0.3 |
3.2 |
4.3 |
|
50 |
0.3 |
3.1 |
4.1 |
|
100 |
0.2 |
2.9 |
3.9 |
|
250 |
0.2 |
2.8 |
3.4 |
|
500 |
0.1 |
2.3 |
2.8 |
Reference Values
|
Analyte |
Units |
Population |
Reference Range |
Critical Value |
|
Total Bilirubin |
mg/dL |
0-6 days |
≥15.0 |
|
|
7-14 days old |
<12.0 |
>15.0 |
||
|
15-28 days old |
0.1-7.0 |
>15.0 |
||
|
|
>28 days |
0.3-1.0 |
|
|
|
Direct Bilirubin |
mg/dL |
All Populations |
0.0-0.2 |
|
Days of Analysis
All
Available STAT
Yes
Includes
Methodology
Beckman AU/Dx Series
CPT Code
82248, 82247
Last Updated
7-Nov-23 BHD